Surgeons remove ‘glowing’ cancer

PURDUE (US) — The first fluorescence-guided surgery on an ovarian cancer patient was performed using a cancer cell “homing device” and imaging agent.

The surgery was one of 10 performed as part of the first phase of a clinical trial to evaluate a new technology to aid surgeons in the removal of malignant tissue from ovarian cancer patients. The method illuminates cancer cells to help surgeons identify and remove smaller tumors that could otherwise be missed.

Plasma microRNA profile to detect ovarian cancer

Summary:

MicroRNAs (miRNA) were discovered that can serve as biomarkers or treatment targets for ovarian cancer. A method was developed to isolate RNA from serum samples and perform a real-time PCR-based microarray for miRNAs using a small sample of serum RNA. RNA was isolated from the serum of patients with ovarian cancer. MicroRNAs (miRNA) were amplified by real-time PCR-based microarray and analyzed. Five miRNAs were overexpressed and three miRNAs were under-expressed in the serum of ovarian cancer patients compared to normal controls. Some of the overexpressed miRNAs are known oncogenes that have therapeutic and biomarker potential.

Potential Applications:

  • Early serum biomarkers for the detection of ovarian cancer (especially for patients with normal CA-125) or risk of ovarian cancer
  • Method for analysis of miRNAs in the serum
  • MicroRNA targets for treatment of ovarian cancer by regulating protein expression by modulating miRNAs

Advantages:

  • RNA extraction from small patient serum samples (250 uL)
  • Novel real-time PCR microarray approach (containing 365 miRNA targets) for identification of microRNAs in RNA samples
  • Microarray platform permits screening of large numbers of miRNAs with small samples of serum RNA (400 ng)
  • Unique profile of microRNAs for the early diagnosis of ovarian cancer was discovered
  • Serum microRNAs were identified that can serve as normalizers