Summary:

Today, one technique used to determine if microRNA expression is regulated post transcriptionally is to measure the levels of the precursor and mature microRNA using sensitive PCR. However, microRNA precursors are difficult molecules to quantify by PCR since they have a great deal of secondary structure.

Researchers at OSU have developed a new methodology to amplify and quantify the levels of microRNA precursors using PCR. PCR detection of both precursor and mature microRNA are needed in order to fully understand the degree of post transcriptional regulation that occurs during development and in various diseases.

Potential Applications:

• Biotechnology supply companies that market PCR reagents, kits and supplies
• In vitro diagnostic based companies
• Scientists interested in studying regulation of microRNA biogenesis

Advantages:

• Sensitive and precise quantification of microRNA precursors.
• Rapid screening of hundreds of microRNA precursors.
• When combined with a PCR assay for mature miRNA, can discover microRNAs that are regulated post transcriptionally.