Summary:

Researchers at the Ohio State University have developed a real-time PCR-based rapid detection system for Alicyclobacillus spp.; the microbe responsible for about 10% of our food supplies lost every year due to food spoilage. Recently this group of bacteria is receiving great attention from food industry, because of its association with spoilage in processed acid foods such as juices and purees. The first probe-and-primer set for PCR is specific for the genus Alicyclobacillus. Using the above method, the presence of less than 100 cells has been detected in a juice system within hours. Rapid detection system for a major foodborne pathogen is also available.

Potential Applications:

Detecting infestations of Alicyclobacillus spp. to prevent food spoilage and food loss.

Advantages:

Fast and reliable method for detecting the presence of contaminating bacteria.